What is transcriptomics and why study it?
Transcriptomics determines which genes are actively expressed, to what extent under specific conditions. With high-throughput sequencing technologies, it provides a comprehensive and quantitative view of gene activity in both prokaryotic and eukaryotic organisms.
Today, transcriptomics is one of the most powerful methods to understand cellular responses to stress, treatments, environmental changes, or developmental stages.
Our RNA-Seq applications
Our services cover all transcriptomic analysis needs:
- Differential expression analysis between conditions or treatments
- Transcript abundance quantification (mRNA)
- Functional gene annotation
- Detection of single nucleotide variants (SNPs) from transcripts
- Multi-omics integration with genomic or metagenomic data
Want to focus on a few genes of interest? We also offer targeted quantification via RT-qPCR, a fast, economical solution.
RNA-Seq Workflow: from sample to results
Experimental design
RNA Extraction
Optimized protocols
Sequencing
Differential expression analysis
Comprehensive Reports (Physical & Summary)
Dedicated scientific team for each project.
Assistance in developing the experimental plan and sequencing strategy
Two methods are used to enrich the RNA fraction of interest (mRNA) prior to cDNA synthesis and library preparation:
- From total eukaryotic RNA: Capture of mRNAs by their polyA tail
- From total prokaryotic or eukaryotic RNA: Depletion of rRNA through capture by complementary probes. Enrichment of samples in mRNA
Comprehensive processing of sequencing data
Differential expression analysis
Functional enrichment analysis
Interactive reports for intuitive exploration of your data:
- Dynamic visualisations (graphs, tables)
- Filtering and sorting tools to target genes or metabolic pathways of interest
Interpretation of results and personalised support for independent use